TAE Buffer 10X (Tris-acetate-EDTA) (Biotechnology Grade)

<strong>TAE Buffer 10X (Tris-acetate-EDTA)</strong>_x000D_ <table width="773">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="140">Catalog number:</td>_x000D_ <td width="633">B2010011</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume:</td>_x000D_ <td>50 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Content</td>_x000D_ <td>Tris-Acetate + EDTA</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>8.3</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance:</td>_x000D_ <td>Clear Solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Application:</td>_x000D_ <td>Agarose DNA electrophoresis (dilute to 1X before use)</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>Two years from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Room temperature</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>TAE solution, tris-acetate EDTA buffer, TAE buffer, DNA electrophoresis buffer, TAE Buffer 10X Solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity <span class="ILfuVd"><span class="e24Kjd">&gt;18 MΩ-cm</span></span>) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="633">1: Strutz K, Stellwagen NC. Do DNA gel electrophoretic mobilities extrapolate to_x000D_ the free-solution mobility of DNA at zero gel concentration? Electrophoresis._x000D_ 1998 May;19(5):635-42. doi: 10.1002/elps.1150190504. PMID: 9629889.2: Stellwagen NC, Bossi A, Gelfi C, Righetti PG. DNA and buffers: are there any_x000D_ noninteracting, neutral pH buffers? Anal Biochem. 2000 Dec 1;287(1):167-75. doi:_x000D_ 10.1006/abio.2000.4848. PMID: 11078596.3: Ungewickell E, Oestergaard L. Identification of the clathrin assembly protein_x000D_ AP180 in crude calf brain extracts by two-dimensional sodium dodecyl sulfate-_x000D_ polyacrylamide gel electrophoresis. Anal Biochem. 1989 Jun;179(2):352-6. doi:_x000D_ 10.1016/0003-2697(89)90143-7. PMID: 2774183.4: Hayes VM, Wu Y, Osinga J, Mulder IM, van der Vlies P, Elfferich P, Buys CH,_x000D_ Hofstra RM. Improvements in gel composition and electrophoretic conditions for_x000D_ broad-range mutation analysis by denaturing gradient gel electrophoresis._x000D_ Nucleic Acids Res. 1999 Oct 15;27(20):e29. doi: 10.1093/nar/27.20.e29. PMID:_x000D_ 10497279; PMCID: PMC148660.5: Stellwagen NC. Apparent pore size of polyacrylamide gels: comparison of gels_x000D_ cast and run in Tris-acetate-EDTA and Tris-borate-EDTA buffers. Electrophoresis._x000D_ 1998 Jul;19(10):1542-7. doi: 10.1002/elps.1150191004. PMID: 9719523.6: Stellwagen E, Stellwagen NC. Determining the electrophoretic mobility and_x000D_ translational diffusion coefficients of DNA molecules in free solution._x000D_ Electrophoresis. 2002 Aug;23(16):2794-803. doi:_x000D_ 10.1002/1522-2683(200208)23:16&lt;2794::AID-ELPS2794&gt;3.0.CO;2-Y. PMID: 12210184.7: Ali RW, Martin L, Haffajee AD, Socransky SS. Detection of identical ribotypes_x000D_ of Porphyromonas gingivalis in patients residing in the United States, Sudan,_x000D_ Romania and Norway. Oral Microbiol Immunol. 1997 Apr;12(2):106-11. doi:_x000D_ 10.1111/j.1399-302x.1997.tb00625.x. PMID: 9227134.8: Common stock solutions, buffers, and media. <a href="https://pubmed.ncbi.nlm.nih.gov/18228281/">Curr Protoc Cell Biol.</a> 2001 May; Appendix 2:Appendix 2A.</td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>_x000D_ <table width="773">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="140">Related Products</td>_x000D_ <td width="633"><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>