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Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial

Product Specifications

Product Name Alternative

AnpAFP

Abbreviation

Recombinant Antarctomyces psychrotrophicus Antifreeze protein (X10S), partial

UniProt

P86268

Expression Region

1-20aa (X10S)

Organism

Antarctomyces psychrotrophicus

Target Sequence

AGLDLGAASSFGALAFEGVA

Tag

N-terminal 6xHis-KSI-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Antifreeze proteins bind to the surface of ice crystals and inhibit the growth of these crystals, this inhibition causes thermal hysteresis. Causes the shape of ice crystals to change from hexagonal to a bipyramidal shape with rugged facets. Inhibits recrystallization of ice crystals.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

17.2 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

Available Sizes

Frequently Asked Questions

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