Methylene blue (hydrate)

Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

Product Specifications

CAS Number

[122965-43-9]

Product Name Alternative

Basic Blue 9 (hydrate) ; CI-52015 (hydrate) ; Methylthioninium (chloride hydrate)

UNSPSC

12352005

Hazard Statement

H302

Target

Guanylate Cyclase; Microtubule/Tubulin; Monoamine Oxidase; NO Synthase

Type

Reference compound

Related Pathways

Cell Cycle/DNA Damage; Cytoskeleton; GPCR/G Protein; Immunology/Inflammation; Neuronal Signaling

Field of Research

Cancer; Infection; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/methylene-blue-hydrate.html

Solubility

H2O : 2 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CN(C1=CC2=[S+]C3=C(C=CC(N(C)C)=C3)N=C2C=C1)C.[x].[Cl-].O

Molecular Formula

C16H18N3S.Cl.xH2O

Precautions

H302

References & Citations

[1]Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94 (2) :65-72. |[2]Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25.|[3]Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32 (2) :127-38.