Monkeypox virus (Democratic Republic of the Congo) A35R Protein, His Tag (MALS verified)
Monkeypox virus (Democratic Republic of the Congo) A35R Protein, His Tag (A3R-M52H4) is expressed from human 293 cells (HEK293) . It contains AA Arg 58 - Thr 181 (Accession # EPI_ISL_19093812, GISAID) .
Product Specifications
Background
Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. The envelope glycoprotein A35R on the EV surface has been predicted to influence intercellular diffusion of virions.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.5 kDa. The protein migrates as 15-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
EPI_ISL_19093812
Expression Region
Arg 58 - Thr 181
Host
HEK293
Target
A35R (MPXV)
Conjugation
Unconjugated
Tag
C-10xHis
Source
Monkeypox virus (Democratic Republic of the Congo)
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Monkeypox virus (Democratic Republic of the Congo) A35R Protein, His Tag (Cat. No. A3R-M52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-A35R Antibody, Human IgG1 (6F11) (Cat. No. A3R-M575) with a linear range of 0.1-3 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
15.5 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
50ug*1
Host or Source
HEK293
Species
Monkeypox virus (Democratic Republic of the Congo)
Protein ID
EPI_ISL_19093812
Preservative
Trehalose
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