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Monkeypox virus (Democratic Republic of the Congo) A35R Protein, His Tag (MALS verified)

Monkeypox virus (Democratic Republic of the Congo) A35R Protein, His Tag (A3R-M52H4) is expressed from human 293 cells (HEK293) . It contains AA Arg 58 - Thr 181 (Accession # EPI_ISL_19093812, GISAID) .

Product Specifications

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. The envelope glycoprotein A35R on the EV surface has been predicted to influence intercellular diffusion of virions.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.5 kDa. The protein migrates as 15-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

EPI_ISL_19093812

Expression Region

Arg 58 - Thr 181

Host

HEK293

Target

A35R (MPXV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Monkeypox virus (Democratic Republic of the Congo)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Monkeypox virus (Democratic Republic of the Congo) A35R Protein, His Tag (Cat. No. A3R-M52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-A35R Antibody, Human IgG1 (6F11) (Cat. No. A3R-M575) with a linear range of 0.1-3 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

15.5 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Monkeypox virus (Democratic Republic of the Congo)

Protein ID

EPI_ISL_19093812

Preservative

Trehalose

Frequently Asked Questions

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