Ac-DEVD-AMC

Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm[1][2][3].

Product Specifications

CAS Number

[169332-61-0]

UNSPSC

12171500

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Applications

Cancer-programmed cell death

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Ac-DEVD-AMC.html

Purity

99.93

Solubility

DMSO : 10 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL

Smiles

CC(C1=CC=C(NC([C@H](CC(O)=O)NC([C@H](C(C)C)NC([C@H](CCC(O)=O)NC([C@@H](NC(C)=O)CC(O)=O)=O)=O)=O)=O)C=C1O2)=CC2=O

Molecular Formula

C30H37N5O13

Molecular Weight

675.64

References & Citations

[1]Nobel CS, et al. Disulfiram is a potent inhibitor of proteases of the caspase family. Chem Res Toxicol. 1997 Dec;10 (12) :1319-24. |[2]Ochu EE, et al. Caspases mediate 6-hydroxydopamine-induced apoptosis but not necrosis in PC12 cells. J Neurochem. 1998 Jun;70 (6) :2637-40. |[3]Yakovlev AA, Gorokhovatsky AY, Onufriev MV, Beletsky IP, Gulyaeva NV. Brain cathepsin B cleaves a caspase substrate. Biochemistry (Mosc) . 2008 Mar;73 (3) :332-6.