CD47 Antibody
CD47 Antibody
Product Specifications
Product Name Alternative
Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6
UniProt
Q08722
Reactivity
Human
Immunogen
Intact CD47 purified from placenta was used as the immunogen for this antibody.
Target
CD47
Clonality
Monoclonal
Clone
B6H12.2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation; Cell Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Functional Activity (Blocking) : Order BSA/Azide-free formatFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 min at RTWestern blot: 2-5 μg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Tested Applications
Blocking, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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