CGB Antibody
CGB Antibody
Product Specifications
Product Name Alternative
CGB, CGB3, CG-beta, Choriogonadotropin beta chain, HCGB, hCG Beta
UniProt
Q6NT52
Reactivity
Human
Immunogen
Recombinant protein was used as the immunogen for this HCG-beta antibody.
Target
CGB
Clonality
Monoclonal
Clone
HCGb/54
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: use BSA-free format (1) IHC (FFPE) : 0.5-1 μg/mL for 30 min at RT (2) Prediluted format : incubate for 30 min at RT (3) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.1. Clone HCGb/54 (recommended detect) will pair with clones HCGb/211 (recommended capture) and HCGb/459 by ELISA.2. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
ELISA, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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