EGF R Antibody
EGF R Antibody
Product Specifications
Product Name Alternative
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
UniProt
P00533
Reactivity
Human
Immunogen
Microsomes from A431 cells were used as the immunogen for this antibody.
Target
EGF R
Clonality
Monoclonal
Clone
B1D8
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Protein G purified monoclonal antibody
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Functional Activity (Inhibition) : antibody blocks EGF receptor activationinhibits A431 cell proliferationarrests tumor growth in vivoaffinity purificationIP: 1-2μg/500ug protein lysateFlow Cytometry: 0.5-1 ug/million cellsThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Tested Applications
FA, FC, IP
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2a, kappa
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