IGHM Antibody

Flow Cytometry: 0.5-1ug/10e6 cellsIF: 0.5-1 μg/mLWB: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Due to differences in protocols and secondaries, the anti-IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.