IGKC Antibody

Product Specifications

UniProt

P01834, P01601

Reactivity

Human

Immunogen

Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.

Target

IGKC

Clonality

Monoclonal

Clone

KLC709

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blot: 0.5-1 μg/mLFlow Cytometry: 0.5-1 ug/million cellsIHC (FrozenFFPE) : 0.5-1.0 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.