INS Antibody

Product Specifications

Product Name Alternative

INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

UniProt

P01308

Reactivity

Bovine, Human, Porcine, Rabbit, Rat

Immunogen

Full length purified pig Insulin (aa 1-84) was used as the immunogen for this antibody.

Target

INS

Clonality

Monoclonal

Clone

E2-E3 or INS04

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.