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PECAM1 Antibody

PECAM1 Antibody

Product Specifications

Product Name Alternative

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

UniProt

P16284

Reactivity

Human, Primate, Rabbit

Immunogen

Human recombinant PECAM-1 / CD31 protein was used as the immunogen for this antibody.

Target

PECAM1

Clonality

Monoclonal

Clone

C31.7

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 μg/mLWB: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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