PECAM1 Antibody
PECAM1 Antibody
Product Specifications
Product Name Alternative
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
UniProt
P16284
Reactivity
Human
Immunogen
Recombinant human protein
Target
PECAM1
Clonality
Monoclonal
Clone
C31.3
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 μg/mL Immunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Differences in protocols, secondaries and substrates may require the CD31 antibody to be titered for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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