PECAM1 Antibody

Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 μg/mL Immunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Differences in protocols, secondaries and substrates may require the CD31 antibody to be titered for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.