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PTPRC Antibody

PTPRC Antibody

Product Specifications

Product Name Alternative

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45

UniProt

P08575

Reactivity

Human

Immunogen

Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.

Target

PTPRC

Clonality

Monoclonal

Clone

PD7/26

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G purified

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western Blot: 0.5-1 μg/mLFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1.0 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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