PTPRC Antibody
PTPRC Antibody
Product Specifications
Product Name Alternative
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
UniProt
P08575
Reactivity
Human
Immunogen
Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.
Target
PTPRC
Clonality
Monoclonal
Clone
PD7/26
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G purified
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western Blot: 0.5-1 μg/mLFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1.0 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
