Golgi Complex Antibody [371-4]

Product Specifications

Reactivity

Human

Immunogen

SU-DHL-1 large cell lymphoma cells were used as the immunogen.

Clonality

Monoclonal

Clone

371-4

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western Blot: 0.5-1 μg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunocytochemistry: (Acetone/paraformaldehyde fixed) 0.5-1 μg/mL for 30 minIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Golgi complex antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.