Mitochondria Marker Antibody [MTC754]

Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLWB: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondria marker antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues is enhanced by boiling sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.