Phosphotyrosine Antibody [PY20]
Phosphotyrosine Antibody [PY20]
Product Specifications
Reactivity
All
Immunogen
Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
PY20
Conjugation
Unconjugated
Field of Research
Cancer Biology, Metabolism Research, Neuroscience, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLWB: 0.5-1 μg/mLIP: 1-2ug/500ug protein lysateIHC (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Tested Applications
FC, IF, IHC, IP, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2b
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