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Phosphotyrosine Antibody [PY20]

Phosphotyrosine Antibody [PY20]

Product Specifications

Reactivity

All

Immunogen

Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

PY20

Conjugation

Unconjugated

Field of Research

Cancer Biology, Metabolism Research, Neuroscience, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLWB: 0.5-1 μg/mLIP: 1-2ug/500ug protein lysateIHC (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.

Tested Applications

FC, IF, IHC, IP, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG2b

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