KLK3 Antibody

ELISA: For coating use Ab at 1-5 μg/mL; order Ab without BSAFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1.0 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.