Her2 Antibody
Her2 Antibody
Product Specifications
Product Name Alternative
ErbB-2 Antibody: NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu, MLN19, NEU, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
UniProt
P04626
Reactivity
Human
Immunogen
Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
Target
Her2
Clonality
Monoclonal
Clone
ERB2/776
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.2. This HER2 antibody is specific to the intracellular region of the protein.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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