CDKN1A Antibody

Product Specifications

Product Name Alternative

Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

UniProt

P38936

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the p21 antibody.

Target

CDKN1A

Clonality

Monoclonal

Clone

CIP1/823

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLWestern Blot: 1-2 μg/mLImmunohistochemistry (FFPE) : 2-4 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the p21 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.