ICAM3 Antibody
ICAM3 Antibody
Product Specifications
Product Name Alternative
Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3
UniProt
P32942
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.
Target
ICAM3
Clonality
Monoclonal
Clone
SPM505
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Functional Activity: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order SAF formulation) Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the anti-ICAM-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
Blocking, FC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2b, kappa
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