IVL Antibody
IVL Antibody
Product Specifications
Product Name Alternative
IVL
UniProt
P07476
Reactivity
Human
Immunogen
Purified involucrin from human keratinocytes was used as the immunogen for the Involucrin antibody.
Target
IVL
Clonality
Monoclonal
Clone
IVRN/827
Conjugation
Unconjugated
Field of Research
Cancer Biology, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 0.1-0.2 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the Involucrin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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