CD34 Antibody
CD34 Antibody
Product Specifications
Product Name Alternative
Hematopoietic progenitor cell antigen CD34, CD34, CD34
UniProt
P28906
Reactivity
Human, Rat
Immunogen
Recombinant full-length human protein was used as the immunogen for the anti-CD34 antibody.
Target
CD34
Clonality
Monoclonal
Clone
HPCA1/1171
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLWestern blot: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 2-4 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the anti-CD34 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC-P, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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