NEFH Antibody

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunohistochemistry (FFPE) : 0.25-0.5 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the Neurofilament antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.