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KRT76 Antibody

KRT76 Antibody

Product Specifications

Product Name Alternative

KRT2B, KRT2P, Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, CK-2P, HUMCYT2A

UniProt

Q01546

Reactivity

Human, Rat

Immunogen

Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.

Target

KRT76

Clonality

Monoclonal

Clone

KRTH/1076

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA (For coating order Ab without BSA) Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLWestern blot: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.25-0.5 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

ELISA, FC, IF, IHC-P, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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