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ENO2 Antibody

ENO2 Antibody

Product Specifications

Product Name Alternative

ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

UniProt

P09104

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Target

ENO2

Clonality

Monoclonal

Clone

ENO2/1375

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blot: 0.5-1 μg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 μg/mLIHC (FFPE) : 0.1-0.2 μg/mL for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Tested Applications

FC, IF, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG2b

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