TOP2A Antibody
TOP2A Antibody
Product Specifications
Product Name Alternative
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
UniProt
P11388
Reactivity
Human
Immunogen
Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
Target
TOP2A
Clonality
Monoclonal
Clone
TOP2A/1362
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 μg/mLWestern blot: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2b, kappa
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