TOP2A Antibody

Product Specifications

Product Name Alternative

DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2

UniProt

P11388

Reactivity

Human, Mouse

Immunogen

Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Target

TOP2A

Clonality

Monoclonal

Clone

TOP2A/1361

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 μg/mLWestern blot: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.