PDCD1 Antibody
PDCD1 Antibody
Product Specifications
Product Name Alternative
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
UniProt
Q15116
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the anti-PD-1 antibody.
Target
PDCD1
Clonality
Monoclonal
Clone
PD203-1
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Immunofluorescence: 1-2 μg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlTitering of the anti-PD-1 antibody may be required for optimal performance.1. FFPE testing requires tissue to be boiled in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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