VIM Antibody
VIM Antibody
Product Specifications
Product Name Alternative
Vimentin, VIM
UniProt
P08670
Reactivity
Human
Immunogen
Full length human recombinant protein was used as the immunogen for the Vimentin antibody.
Target
VIM
Clonality
Monoclonal
Clone
VIM66-1
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Neuroscience, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blot: 1-2 μg/mLIHC (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the Vimentin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, k
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