PCNA Antibody
PCNA Antibody
Product Specifications
Product Name Alternative
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
UniProt
P12004
Reactivity
Human
Immunogen
Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.
Target
PCNA
Clonality
Monoclonal
Clone
PM441-1
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blot: 1-2 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the PCNA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2a, kappa
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