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CD44 Antibody

CD44 Antibody

Product Specifications

Product Name Alternative

CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglyc

UniProt

P16070

Reactivity

Human, Mouse

Immunogen

Purified human protein was used as the immunogen for the CD44 antibody.

Target

CD44

Clonality

Monoclonal

Clone

HCM15-1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the CD44 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IHC-P

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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