GFAP Antibody

Product Specifications

Product Name Alternative

Glial fibrillary acidic protein, GFAP, GFAP

UniProt

P14136

Reactivity

Human

Immunogen

Native Glial fibrillary acidic protein was used as the immunogen for this GFAP antibody.

Target

GFAP

Clonality

Monoclonal

Clone

GFA12-3

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blot: 1-2 μg/mLIHC (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the GFAP antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.