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Mitochondrial Antibody [MTL56]

Mitochondrial Antibody [MTL56]

Product Specifications

Reactivity

Human

Immunogen

Human mitochondria were used as the immunogen for this Mitochondrial antibody.

Clonality

Monoclonal

Clone

MTL56

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) IF: 0.5-1 μg/mLWestern blot: 1-2 μg/mLTitering of the Mitochondrial antibody may be required for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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