Nucleoli Marker Antibody [NCM102-1]

Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1.0 μg/mLImmunocytochemistry (Acetone-fixed) : 0.5-1.0 μg/mL for 30 min at RTIHC (Formalin-paraffin) : 0.5-1.0 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the Nucleoli marker antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.