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KRT8 Antibody

KRT8 Antibody

Product Specifications

Product Name Alternative

KRT8, CARD2, CK8, CYK8, K2C8, K8, KO, CK-8

UniProt

P05787

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this Cytokeratin 8 antibody.

Target

KRT8

Clonality

Monoclonal

Clone

CYKN8-1

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: 1-2 μg/mLIF: 1-2 μg/mLIHC (FFPE) : 0.5-1.0 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1

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