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ESR1 Antibody

ESR1 Antibody

Product Specifications

Product Name Alternative

ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER

UniProt

P03372

Reactivity

Human

Immunogen

Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.

Target

ESR1

Clonality

Monoclonal

Clone

NR3Ga-3

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blot: 1-2 μg/mLFlow Cytometry: 0.5-1 ug/million cellsIHC (Formalin-paraffin) : 1-2 μg/mL (1) Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.1. FFPE testing requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min and cooling at RT for 20 minutes.

Tested Applications

FC, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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