PTPRC Antibody

Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 μg/mLImmunohistology (FFPE) : 0.5-1.0 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the anti-CD45RA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.