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FBXO5 Antibody

FBXO5 Antibody

Product Specifications

Product Name Alternative

F-box only protein 5, Early mitotic inhibitor 1, FBXO5, EMI1, FBX5

UniProt

Q9UKT4

Reactivity

Human

Immunogen

A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Target

FBXO5

Clonality

Monoclonal

Clone

FBXP5

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blot: 1-2 μg/mLImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC-P, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG2a, kappa

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