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IVL Antibody

IVL Antibody

Product Specifications

Product Name Alternative

IVL

UniProt

P07476

Reactivity

Human

Immunogen

Purified protein was used as the immunogen for the Involucrin antibody.

Target

IVL

Clonality

Monoclonal

Clone

INVO49

Conjugation

Unconjugated

Field of Research

Cancer Biology, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 0.1-0.2 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the Involucrin antibody may be required for optimal performance.1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37oC. Enzyme digestion is more effective than Citrate buffer based epitope unmasking.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC-P

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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