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CD99 Antibody

CD99 Antibody

Product Specifications

Product Name Alternative

CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 molecule, E2 antigen, HBA71

UniProt

P14209

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the CD99 antibody.

Target

CD99

Clonality

Monoclonal

Clone

CDLA99

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the CD99 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Not suitable for rat samples testing.

Tested Applications

FC, IF, IHC-P

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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