TAGLN Antibody

Product Specifications

Product Name Alternative

Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, TAGLN, SM22, WS3-10

UniProt

Q01995

Reactivity

Human, Mouse

Immunogen

Recombinant full-length human protein was used as the immunogen for the SM22 alpha antibody. (3)

Target

TAGLN

Clonality

Monoclonal

Clone

SMP22a

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the SM22 alpha antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Cross-reaction with SM22 beta protein has not been determined.