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FSCN1 Antibody

FSCN1 Antibody

Product Specifications

Product Name Alternative

Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55, FSCN1, FAN1, HSN, SNL

UniProt

Q16658

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Target

FSCN1

Clonality

Monoclonal

Clone

FAN55-1

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunofluorescence: 1-2 μg/mLWestern blot: 1-2 μg/mLImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC-P, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG2a, kappa

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