ZAP70 Antibody
ZAP70 Antibody
Product Specifications
Product Name Alternative
Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK
UniProt
P43403
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the ZAP-70 antibody.
Target
ZAP70
Clonality
Monoclonal
Clone
ZTP70-1
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the ZAP-70 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2a, kappa
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