CCNB1 Antibody
CCNB1 Antibody
Product Specifications
Product Name Alternative
G2/mitotic-specific cyclin-B1, CCNB1, CCNB
UniProt
P14635
Reactivity
Human, Mouse
Immunogen
Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
Target
CCNB1
Clonality
Monoclonal
Clone
BCLB1-1
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the Cyclin B1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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