HSP90B1 Antibody
HSP90B1 Antibody
Product Specifications
Product Name Alternative
Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1
UniProt
P14625
Reactivity
Human
Immunogen
Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.
Target
HSP90B1
Clonality
Monoclonal
Clone
SRPR90b
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blot: 1-2 μg/mLImmunofluorescence: 1-2 μg/mLImmunohistology (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the GRP94 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC-P, WB
Host or Source
Rat
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2a, kappa
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