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HSP90B1 Antibody

HSP90B1 Antibody

Product Specifications

Product Name Alternative

Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1

UniProt

P14625

Reactivity

Human

Immunogen

Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

Target

HSP90B1

Clonality

Monoclonal

Clone

SRPR90b

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Western blot: 1-2 μg/mLImmunofluorescence: 1-2 μg/mLImmunohistology (FFPE) : 1-2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the GRP94 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC-P, WB

Host or Source

Rat

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG2a, kappa

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