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DsDNA Antibody [DRNC1-1]

This is a purified, unconjugated, monoclonal antibody suitable for ICC, IF and IHC-P. Cell nuclei were used as the immunogen for the dsDNA antibody.

Product Specifications

Reactivity

Human

Immunogen

Cell nuclei were used as the immunogen for the dsDNA antibody.

Clonality

Monoclonal

Clone

DRNC1-1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunofluorescence: 1-2 μg/mLImmunocytochemistry (Acetone-fixed) : 0.5-1 μg/mL for 30 min at RTImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Titering of the dsDNA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

ICC, IF, IHC-P

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG3, kappa

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