DsDNA Antibody [DRNC1-1]
This is a purified, unconjugated, monoclonal antibody suitable for ICC, IF and IHC-P. Cell nuclei were used as the immunogen for the dsDNA antibody.
Product Specifications
Reactivity
Human
Immunogen
Cell nuclei were used as the immunogen for the dsDNA antibody.
Clonality
Monoclonal
Clone
DRNC1-1
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunofluorescence: 1-2 μg/mLImmunocytochemistry (Acetone-fixed) : 0.5-1 μg/mL for 30 min at RTImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Titering of the dsDNA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
ICC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG3, kappa
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